Creative Bioarray has been committed to cell biology technology research for many years. Based on advanced technical equipment and complete platform construction, we can provide customers with qualitative analysis and detection services for studying cell death.
Cell death is always involved in cell experiments, presenting problems during certain tests. Non-specific staining of dead cells will increase the imaging background and affect the sensitivity of detection. The staining of dead cells will also affect the correct ratio of results. Therefore, quantitatively detecting death cells is very important.
Creative Bioarray has established a complete cell culture platform, equipped with complete animal experiment-related detection and analysis equipment. We can provide customers with a variety of basic cytological experiments. Our services include but are not limited to:
Trypan blue is a blue cell viability dye that cannot enter the cell interior through a complete cell membrane. After the cell dies, the stability of the cell membrane is lost, and trypan blue can enter the cell and stain the cell blue. Therefore, in cell experiments, it is often used to distinguish live cells from dead cells, and to detect the integrity of cell membranes.
Figue 1. Trypan blue staining principle
Living cells / dead cells fixable stains are fixable viability dyes that help to ensure accurate assessment of cell viability in samples after fixation and/or permeabilization.
This method is based on the reaction of fluorescent reactive dyes with cellular proteins. These dyes cannot penetrate the membranes of living cells, so only the proteins on the cell surface can react with the dyes to produce weak staining. These reactive dyes can penetrate the damaged cell membranes of dead cells and stain the internal and external amines to achieve brighter staining.
Figue 2. Living cells / dead cells fixable staining
The time depends on the experiment content
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