Detection of p62/SQSTM1

In addition to LC3 protein, p62 or other linker molecules can also be used as markers of autophagy. Based on the characteristic of autophagy adaptor protein degradation through a selective autophagy pathway, Creative Bioarray can provide you with accurate and efficient autophagy detection.

Background

In addition to LC3, levels of p62 (also known as SQSTM1/sequestosome 1) can also be used to monitor autophagic flux. p62 possesses a C-terminal ubiquitin-binding domain and a short LC3-interacting region sequence. It serves as an adaptor protein that links ubiquitinated proteins to the autophagic machinery and enables their clearance in the lysosome. p62 and p62-bound ubiquitinated proteins become incorporated into the completed autophagosome and are degraded in autolysosomes. Since the intracellular content of such adaptor molecules is obviously regulated by the level of autophagy, the protein level of adaptor molecules in the cell can reflect the autophagy flow in the cell. P62 is mainly removed by autophagy, and its amount is generally considered to be inversely proportional to autophagy activity.

Our Services

There are many methods for studying autophagy based on the level of P62/SQSTM1. Creative Bioarray focuses on technological improvement and innovation, and can provide you with more accurate and efficient research services. Our services include but are not limited to:

P62/SQSTM1-luciferase Testing Service

Creative Bioarray has improved the P62/SQSTM1-based detection method by constructing a cell line stably expressing SQSTM1-luc protein, which is more suitable for throughput screening.

We constructed a recombinant plasmid expressing the selective autophagy substrate p62/SQSTM1 (Sequistosome1) and the firefly luciferase reporter gene. By detecting the activity of intracellular luciferase, autophagy flow can be analyzed.

Experiment Process

Experiment process - Creative Bioarray

Advantages

  • No need for autophagic flux inhibitors
  • Accurate and reliable test results
  • Simple experiment operation
  • Suitable for throughput and engineering screening
  • Can be detected with ordinary luminescence detector

Customer Notice

Customers provide

  • Samples
  • The choice of experiment types
  • Test purposeand requirements

We deliver

  • High-quality test reagents
  • Experiment process
  • Photos and relevant analysis data
  • Complete experiment report

Experiment cycle

The time depends on the experiment content

Advantages of Our Services

  • Complete instrument platform

    We have many types of microscopes and other instruments with high resolution to provide you with clear and high-quality pictures.

  • High standard

    The experimenters have many years of successful experiment experience and can guarantee the standard of experiment operation and experiment process.

  • Short time and low cost

    We provide customers with the most comprehensive services at the most favorable price and help customers improve the efficiency of research.

  • Safety

    All experiments have signed confidentiality agreements, focusing on protecting customer privacy.

If you are interested in our services, please contact us for more detailed information.

* For scientific research only