The accidental death of cells occurs when they are damaged or subjected to physical trauma. Pyroptosis is a recently discovered pathway of host cell death. Creative Bioarray can provide customers with immunofluorescence staining services of GSDMD/GSDME to accurately identify pyroptosis.
Pyroptosis is a process of programmed inflammatory cell death. Compared with apoptosis, pyroptosis occurs faster and is accompanied by the release of a large number of pro-inflammatory factors. It causes shearing and multimerization of various Gasdermin family members including GSDMD, causing cell perforation, and then cell death.
Main features of pyroptosis:
1. Rupture of the cell membrane.
2. Random degradation of DNA.
3. The production of inflammatory bodies.
4. The release of inflammatory cytokines IL-1β and IL-18.
Pyrocytosis is widely involved in the occurrence and development of various diseases. Therefore, the study of cell pyroptosis helps to understand its role in related diseases and provides new ideas for clinical treatment. Our services include but are not limited to:
Studies have shown that GSDMD is closely related to the formation of holes in the cell membrane. Gasdermin D is composed of 480 amino acids and can be divided into two functional domains: gasdermin-N domain at the N-terminus and gasdermin-C domain at the C-terminus. The two domains are connected by a long loop.Caspase-1 and caspase-11 (caspase-4, caspase-5 in humans) can cut Gasdermin D from the loop ring, and the gasdermin-N domain can form 1-2nm pores on the cell membrane surface, thereby promoting the occurrence of pyrolysis.
Creative Bioarray can use fluorescent immunostaining to detect Gasdermin D to help you identify pyroptosis.
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