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Cell Pyroptosis Assay

Introduction

Pyroptosis, also known as inflammatory necrosis, is a kind of programmed cell death. The main feature is that the cells continue to swell until the cell membrane ruptures, resulting in the release of cell contents and activating a strong inflammatory response.

Studies have shown that pyroptosis is widely involved in the occurrence and development of infectious diseases, nervous system-related diseases and atherosclerotic diseases.

The in-depth study of pyroptosis will help to understand its role in the occurrence, development and outcome of related diseases, and provide new ideas for clinical prevention and treatment.

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The morphological characteristics, occurrence and regulation mechanism of pyroptosis are different from other cell death methods such as apoptosis and necrosis. Pyroptosis is programmed cell necrosis mediated by gasdermin D (GSDMD). Once this happens, the N-terminus of the GSDMD protein polymerizes and binds to lipids, forming holes in the cell membrane. The cells gradually swell until the cells rupture, and finally a large amount of cell content such as IL-1β is released, which activates a strong inflammatory response.

There are many methods for detecting pyroptosis:

Detect morphological changes
  • Scanning electron microscope to observe cell morphology
  • TUNEL staining
  • Immunofluorescence staining (GSDMD/GSDME)
Detection of pyrolysis-related proteins
  • qPCR/Western Blot method to detect the expression level of Pyroptosis-related Genes or Proteins.
  • ELISA to detect the level of inflammatory factors.
  • MTT method to determine cell viability.

Creative Bioarray provides you with a variety of pyroptosis detection methods.

Service Process

Creative Bioarray has a professional testing platform and an experienced scientific research team. We can provide scientific researchers with one-stop detection services of pyroptosis.

  • Evaluation of experimental projects
  • Formulation of experimental protocol
  • Preparation of experimental samples and reagents
  • Experimentation
  • Result analysis
  • Delivery of laboratory reports containing raw data, images, and results
  • After-sales service

Service Process

Notice

Experiment contentDelivery contentExperiment cycle
Detection of the Expression Level of Pyroptosis -related Genes or ProteinsPictures, raw data and experimental reportThe time depends on the experiment content
Pyroptosis Related Inflammatory Factors Expression Level DetectionRaw data and experimental report
Immunofluorescence Staining of GSDMD/GSDMEPictures and experimental report

We promise to provide objective and unbiased experimental results, but due to the uncertainty of scientific experimental results, we do not make any commitments as to whether the experimental results meet expectations.

Why Choose Us

  • One-stop technical service

    We provide one-stop services such as cell culture, cell staining and photo taking.

  • Professional

    We have a professional technical team, they have rich operating experience and superb technology, can ensure that the inspection is completed efficiently and accurately.

  • High quality

    We have high-quality and rich product lines, which can provide you with high-sensitivity and high-specificity apoptosis detection reagents.

  • Personalized service

    We provide a variety of apoptosis detection methods to meet customers' individual scientific research needs.

  • Short time and low cost

    We provide customers with the most comprehensive services at the most favorable price and help customers improve the efficiency of research.

  • Safety

    All experiments have signed confidentiality agreements, focusing on protecting customer privacy.

If you are interested in our services, please contact us for more detailed information.

* For scientific research only

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