Autophagy is a life phenomenon widely existing in eukaryotic cells. It is a type of protein degradation pathway that depends on lysosomes and is very conserved in eukaryotes. When cells experience various signals from their environments, such as the lack of nutrients, amino acids and sugars, changes in pH or osmotic pressure, autophagy can make cells respond to stress and survive in harsh environments. At the same time, the process of autophagy removes misfolded or aggregated proteins in the cell and damages or ages organelles to maintain the homeostasis of the cell. When autophagy occurs, the cytoplasmic components inside the cell are encapsulated in autophagosomes, and autophagosomes fuse with lysosomes for degradation, producing new small molecules, such as amino acids, for cell reuse.
Many researchers are currently looking into autophagy as it is widely involved in various physiological and pathological processes. A series of studies have found that the signal pathway of autophagy is very complicated. It has been reported that 40 autophagy-related proteins (Atg proteins) are involved in the formation of autophagosomes. Currently commonly used autophagy detection methods include electron microscopy, immunofluorescence, western blotting and other methods to detect autophagosomes and their marker proteins. An accurate and comprehensive assessment of autophagy includes not only the detection of autophagosomes, but also the dynamic observation of the entire autophagic degradation process. Any method alone cannot be used as a basis for autophagy study.
As a leading manufacturer and provider of biological reagents, Creative Bioarray has been committed to the development of the most advanced experimental reagents to help research on autophagy. We provide a series of products to study autophagy activity, which can be applied to imaging detection platforms and microplate detection platforms. Our products can be used to track the process of lysosome incorporation into autophagosomes, and can also detect the whole process of protein synthesis to aggregation and ubiquitination through the autophagy pathway. Our products include but are not limited to monoclonal antibodies, polyclonal antibodies, labeled antibodies, proteins, immunoassays, and small molecule activators, inhibitors, etc.
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