NLRC4 Inflammasome-dependent Cell Pyroptosis Assay

NLRC4 inflammasome can induce the maturation and secretion of pro-inflammatory factors IL-1β and IL-18 by recruiting and activating caspase-1, and can also induce pyroptosis. NLRC4 inflammasome is involved in the body's innate immune response and plays an important role in immune regulation during infection with a variety of pathogens.

Creative Bioarray can provide customers with inducing NLRC4 inflammasome activation services and detection services of ASC, NLRC4, caspase-1, GSDMD, IL-1β, IL-18 and other proteins or genes. We aim to help customers better study NLRC4 inflammasome-dependent pyroptosis.


NLRC4 inflammasome is associated with auto-inflammatory diseases.

Flagellin, a component of bacterial flagella, plays an important role in the activation of the NLRC4 inflammasome, which can also be activated by aflagellate bacteria such as Shigella flexneri. NLRC4 inflammasome present in macrophages, neutrophils, and intestinal epithelial cells mediates substrate cleavage via CASP1 or CASP8 to induce effector programs, including the release of inflammatory mediators and pyroptosis. A growing number of researchers are discovering an important role for NLRC4 in driving auto-inflammation in humans. The foundations of NLRC4 biology, from monomeric structure to human phenotype, provide important insights and tools for exploring innate immune mechanisms.

Structural, functional, and translational insights gleaned from the study of NLRC4
Structural, functional, and translational insights gleaned from the study of NLRC4 inflammasome.
(Duncan JA, et al.,2018)

Our Services

Our services mainly include in vitro induction of NLRC4 inflammasome activation and NLRC4-dependent pyroptosis.

  • Induce NLRC4 inflammasome activation in vitro

The first step (establishing in vitro models):

Classic in vitro models of NLRC4 inflammasome activation include THP-1 and BMDM. We first induce THP-1 from monocytes to appropriate macrophages with PMA, or we isolate bone marrow cells from the femur and tibia of mice and induce them to BMDM.

Second step (induce NLRC4 inflammasome activation):

We complete the priming process by treating cells with LPS for several hours, followed by the addition of S. typhimurium or flagellin to stimulate NLRC4 inflammasome activation.

  • NLRC4-dependent pyroptosis detection

Creative Bioarray uses methods such as PCR, Western Blot, and ELISA to help you detect the expression levels of pyroptosis-related genes or proteins during NLRC4 inflammasome activation, such as NLRC4, caspase-1, ASC, GSDMD, IL-1β , IL-18 etc., ASC spot analysis can also be performed by confocal microscopy.

Customer Notice

Customers provide

• (Optional, not required) Experimental samples, such as cells or cell culture supernatant

• Research purpose and requirements

• Choice of experiment type

We deliver

In vitro NLRC4 inflammasome activation model

• High quality induction reagents, detection reagents and antibodies

• Experiment process

• Complete experiment report

Experiment cycle

The time depends on the experiment content.

Advantages of Our Services

  • Personalized

We can assist you to complete the design of the experimental content according to the experimental ideas, experimental literature, purpose and requirements you provide.

  • Multiple choices

We provide you with comprehensive pyroptosis detection methods, you can choose according to your needs.

  • Short time and low cost

We provide customers with the most comprehensive services at the most favorable price and help customers improve the efficiency of research.

  • Safety

All experiments have signed confidentiality agreements, focusing on protecting customer privacy.

If you are interested in our services, please contact us for more detailed information.

* For scientific research only