High-Content Screening

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Drug Discovery

High-Content Screening (HCS)

HCS is the process of screening large libraries of compounds or genetic manipulations on cells or organisms using automated imaging systems. This allows for the rapid and systematic evaluation of the effects of these compounds or genetic manipulations on various cellular processes. HCS can be used to study a wide range of cellular parameters, such as cell morphology, protein localization, cell proliferation, and cell death.

High-Content Analysis (HCA)

HCA refers to the analysis of the data generated from HCS experiments. It involves the use of advanced image analysis algorithms and statistical techniques to extract quantitative information from the images. This information can then be used to identify hits, classify different cell phenotypes, or study the effects of compounds on specific cellular pathways. HCA allows for the extraction of more detailed and quantitative data compared to traditional manual microscopy techniques.

In summary, HCS is the experimental part of the process that involves automated imaging and data collection, while HCA is the computational and analytical part that involves extracting meaningful information from the acquired data. Together, they form a powerful approach for studying cellular responses in a high-throughput manner, particularly in the context of drug discovery, functional genomics, and other cell biology applications.

The combination of 2D/3D cell models with HCS & HCA enables researchers to assess the effects of drugs or other compounds in a more biologically relevant context. Creative Bioarray's team of scientists and experts collaborate closely with clients to tailor and refine 2D/3D cell-based high-content screening (HCS) protocols, integrating them with high-content analysis (HCA). This combination empowers researchers to evaluate the impact of drugs or other substances in a biologically meaningful setting. Our comprehensive and top-notch support spans the entirety of the experiment, encompassing study design, execution, and data analysis.

Highlights of Creative Bioarray's High-Content Screening Assay

  • Screen in 2D and 3D cell models, spheroids or organoids
  • Rapid analysis of large numbers of samples
  • Detailed observation of cellular events
  • Temporal analysis of live cells
  • Numeric data on individual cells
  • Automated high-speed imaging and analysis

Flow Chart of High-Content Screening Assay

Flow Chart of High-Content Screening Assay

Diverse Cell Types & Cell Models

  • 2D cell models
    • Cancer cell lines
    • Stem cells (hESC, iPSC)
    • Primary cells
    • Genome engineered cells
  • 3D cell models (e.g. tumor spheroids, organoids)

Available Assays

  • Cytotoxicity

Cell Model Type:

  • 2D cell models
  • 3D cell models (e.g. tumor spheroids, organoids)

Cell Types Available:

  • Cancer cell lines
  • Primary cells
  • iPSC derived organoid
    * Client provided cells or custom models are available on request

Markers:

  • Molecular Probes: LIVE/DEAD fixable dead cell probe
  • Total cell count: DAPI
    * Other dead cell probes are available on request
  • Apoptosis

Cell Model Type:

  • 2D cell models
  • 3D cell models (e.g. tumor spheroids, organoids)

Cell Types Available:

  • Cancer cell lines
  • Primary cells
  • iPSC derived organoid
    * Client provided cells or custom models are available on request

Markers:

  • Molecular Probes: Apoptosis probe
  • Total cell count: DAPI
    * Other apoptosis probes available on request
  • Antibody Pharmacokinetics

Cell Model Type:

  • 3D cell models (e.g. tumor spheroids, organoids)
  • Tissue biopsy/necropsy

Cell Types Available:

  • Cancer cell lines
  • Primary cells
    * Client provided cells or tissue biopsies/necropsies
    * Custom models available on request

Markers:

  • DAPI
* For scientific research only

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