Cell migration plays a pivotal role in tumor development, serving critical functions in angiogenesis for ensuring tumor nutrition and facilitating the formation of metastases as tumor cells migrate from the primary site to invade other tissues. This dynamic process is triggered by diverse agents, including growth factors and chemokines, involving intricate signaling events that engage various components of the cellular motility machinery. Key signaling components such as FAK, cSrc, and ROCK, along with ligand/receptor interactions that drive migration, emerge as promising targets for therapeutic interventions in cancer treatment.
To devise effective therapies inhibiting tumor cell migration, a comprehensive understanding of the stimuli-driven mechanisms in both tumor cells and their microenvironment is essential. Utilizing cell migration assays, such as transwell migration assay, the classical scratch or wound healing assay, and Oris assay, provides insights into the speed and characteristics of migration, particularly following interventions like gene knockdowns or drug treatments. For studies requiring measurement of directed cell migration towards a chemoattractant, conducting a chemotaxis assay is warranted.
Creative Bioarray's tumor cell biology teams offer extensive expertise in the development and execution of cell migration assays, catering to both standard compound screening and customized solutions.