Co-culture models are a valuable tool for studying metabolic bone diseases as they allow for the investigation of interactions between different cell types and tissues that are important for bone health.
Osteoblasts are bone-forming cells responsible for the synthesis and mineralization of bone matrix, while osteoclasts are bone-resorbing cells responsible for the breakdown and resorption of bone tissue. In this co-culture system, osteoblasts and osteoclasts are cultured together to mimic the complex interactions that occur between these two cell types in vivo.
Figure 1. Various technique of co-culture in in vitro model of bone remodeling process.[1]
The co-culture system allows researchers to investigate the crosstalk between osteoblasts and osteoclasts and how it regulates bone remodeling processes such as bone formation and resorption. It can also be used to study the effects of various factors, such as hormones, cytokines, and drugs, on osteoblast-osteoclast interaction.
Creative Bioarray's OB-OC Co-culture System provides a powerful tool for studying the dynamic interplay between osteoblasts and osteoclasts, and offers a valuable platform for drug discovery and development in the field of bone biology.
Study Examples:
Figure 2. Triple culture setup for patterned and mixed seeding of OC and OB (A) OB and OC on the porous membrane after patterned seeding (B) as well as mixed seeding (C) and 7 days of triple culture. Fixed cells were stained with Alexa fluor 488 phalloidin and DAPI to stain cytoskeleton (green) and nuclei (blue). [2]
References:
1. Azizul, N., Hapidin, H., Abdullah, H., Azlan, M., Ahmad, A., & Soelaiman, I. (2023). "Potential Effects of Polyphenols on Osteoblast and Osteoclast Culture." Biomedical Research and Therapy, 10(1), 5476-5490. https://doi.org/10.15419/bmrat.v10i1.786
2. Bernhardt, Anne et al. "Triple Culture of Primary Human Osteoblasts, Osteoclasts and Osteocytes as an In Vitro Bone Model." International journal of molecular sciences vol. 22,14 7316. 7 Jul. 2021, doi:10.3390/ijms22147316
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