The pyroptosis caused by the activation of NLRP3 inflammasome is a self-protection measure of the body, which can help resist exogenous microbial infection and endogenous cell damage, and maintain its own internal environment homeostasis.
Creative Bioarray can provide customers with NLRP3 inflammasome activation services and ASC, NLRP3, caspase-1, GSDMD, IL-1β, IL-18 and other protein or gene testing services. Creative Bioarray is committed to assisting customers to better study NLRP3 inflammasome-dependent cell pyroptosis.
NLRP3 inflammasome is a group of cytoplasmic protein complexes, which are composed of NLRP3, ASC and caspase-1. The activation of NLRP3 inflammasomes in macrophages requires two steps: priming and activation. The priming step (signal 1) is provided by inflammatory stimuli, such as TLR4 agonists, which induce NF-κB-mediated expression of NLRP3 and pro-IL-1β. The activation step (signal 2) is triggered by PAMPs and DAMPs to promote NLRP3 inflammasome assembly. The assembled NLRP3 inflammasome can activate the protease caspase-1 to induce Gasdermin D-dependent pyroptosis and promote the maturation and release of IL-1β and IL-18, which contributes to the maintenance of innate immune defense and homeostasis.
Priming and activation of the NLRP3 inflammasome. (Yu ZW, et al., 2019)
At the same time, NLRP3 inflammasome is also involved in the occurrence and development of diabetes, atherosclerosis, gout and other diseases, making clinical symptoms and treatment more complicated. Studying the mechanism of NLRP3 inflammasome activation will help develop its specific inhibitors to treat NLRP3-related diseases.
Our services mainly include in vitro induction of NLRP3 inflammasome activation and NLRP3-dependent pyroptosis detection.
The first step (establishment of in vitro models):
Classical in vitro models of NLRP3 inflammasome activation include THP-1 and Bone marrow-derived macrophages (BMDM). We first used PMA to induce THP-1 from monocytes into suitable macrophages, or isolated bone marrow cells from mouse femurs and tibias, and induced them into BMDM cells.
The second step (induction of NLRP3 inflammasome activation):
The priming process is initiated by LPS, leading to the activation of the transcription factor NK-κB and the subsequent transcription and synthesis of pro-IL1β and NlRP3. The activation process is induced by specific NLRP3 stimuli such as Nigericin, ATP, MSU, ALUM, etc., causing the assembly and activation of NLRP3 inflammasomes, and mediating the autocatalytic activation of Caspase-1. Active caspase-1 will further cause pro-IL -1β and pro-IL-18 maturation and secretion.
Creative Bioarray uses PCR, Western Blot, ELISA and immunofluorescence detection methods to help you detect the expression level of pyrolysis-related genes or proteins during the activation of NLRP3 inflammasomes, such as ASC, NLRP3, caspase-1, GSDMD, IL-1β, IL-18, etc.
The research team have many years of successful experiment experience and can guarantee the standard of experiment operation and experiment process.
We provide customers with the most comprehensive services at the most favorable price and help customers improve the efficiency of research.
All experiments have signed confidentiality agreements, focusing on protecting customer privacy.
If you are interested in our services, please contact us for more detailed information.
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