Cytotoxicity analysis can be used in basic research and drug discovery to screen toxic compound libraries. There are many commonly used cytotoxicity detection techniques. Creative Bioarray provides customers with multiple options for cytotoxicity testing services.
Cytotoxicity is a simple cell killing event caused by synthetic compounds, natural toxic or immune regulatory cells (such as cytotoxic T lymphocytes, natural killer cells). Cytotoxicity does not refer to a specific cell death mechanism, and does not depend on the cell death mechanism of apoptosis or necrosis. Cytotoxicity is an adverse reaction leading to disorders of cell functions.
Cytotoxicity can be divided into 3 types according to the mechanism of action:
Creative Bioarray has developed a series of cytotoxicity research methods. We can undertake various types of cell cytotoxicity testing and provide a full set of customized technical services such as cell culture, cell processing, image collection, and data measurement. Our services include but are not limited to:
MTT Method
2,5-diphenyltetrazolium bromide (MTT) can penetrate the cell membrane into the cell. Succinate dehydrogenase in the mitochondria of living cells can reduce MTT to purple formazan and deposit it in the cell. However, dead cells do not have this function. Dimethyl sulfoxide can dissolve formazan in cells. The light absorption value can be measured with an enzyme-linked immunoassay at 490nm wavelength. Within a certain range, the amount of MTT crystal formation is proportional to the number of cells. According to the measured absorbance value (OD value), the degree of cell proliferation is determined. The MTT method is simple, fast and accurate, and is often used in cytotoxicity tests.
Advantagesof MTT Method
CCK-8 Method
CCK-8 (Cell Counting kit-8) reagent contains WST-8, which is a compound similar to MTT. In the presence of electronic coupling reagents, it can be reduced by some dehydrogenases in mitochondria to produce orange-yellow formazan. The color intensity has a linear relationship with the number of cells: The faster the cell proliferation, the darker the color; the more cytotoxic, the lighter the color. Compared with the MTT, the CCK-8 has higher detection sensitivity, the product is more soluble, and the detection result is more stable.
Advantagesof CCK-8 Method
MTT/CCK-8 Experiment Process
MTT/CCK-8 Method Applications
LDH (lactate dehydrogenase) is an enzyme that exists in the cytoplasm. When the cell membrane is damaged, LDH is released into the culture medium. The amount of LDH in the detection medium can be used as an indicator to determine the number of dead and damaged cells. By detecting the activity of LDH in the cell culture supernatant, the degree of cell damage can be determined. This analysis is sensitive, convenient, accurate and has a wide range of applications.
In addition, when a cell is damaged, a variety of substances that only exist in the cell (such as adenylate kinase GAPDH, lactate dehydrogenase LDH, etc.) will be released outside the cell. Creative Bioarray can couple the enzymatic reaction of these enzymes with the luminescence of luciferase, and determine cytotoxicity through quantitative bioluminescence and multi-well plate detection. Compared with the colorimetric method and the fluorescence method, the bioluminescence method is more sensitive and can accurately detect the LDH released from a small number of cells, primary cells and 3D cells.
Advantagesof Cytotoxicity LDH Assay
The time depends on the experiment content
If you are interested in our services, please contact us for more detailed information.
Online Inquiry
