Comprehensive Solutions for Autophagy

Introduction

What Is Autophagy

Autophagy is a lysosomal degradation pathway in which damaged or excess cell components are degraded and then recycled back to the cytoplasm. In this respect, autophagy drives a flow of biomolecules in a continuous degradation-regeneration cycle. Autophagy is generally considered to be a self-protection mechanism that protects cells under stress or malnutrition conditions. Current research has proved that autophagy has many functions in important biological processes. It is closely related to development, differentiation, innate and adaptive immunity, aging and cell death.

Autophagy and Diseases

As autophagy plays a role in many important biological processes, defects in autophagy signaling could be associated with many diseases. Autophagy has been linked to neuronal disorders, liver and heart diseases, infectious diseases, cancer, diabetes type II, cystic fibrosis and many more. 

Solutions for You

Creative Bioarray has a professional autophagy research platform. The main methods of autophagy detection include but are not limited to:

Morphological Feature Detection

• Transmission electron microscope observation
• Trace autophagy formation
• Fluorescence staining

Autophagy Marker Protein Detection

We provide autophagy marker detection services to detect cytotoxicity marked by cell viability and apoptosis marked by caspase activation to determine the mechanism of cell death. 

• Autophagy marker protein LC3
• Detection of autophagy substrate proteins
• Atg12-Atg5 complex
• Beclin1
• Marker proteins related to autophagy also include ATG1, ATG9, DRAM1, ATG14, ATG16 and ATG18.

Autophagy Compound Library and Small Molecule Screening

Autophagy compound library is a useful tool for screening autophagy antibodies in vitro. The compound library includes a variety of autophagy inducers or inhibitors, which can screen autophagy-modulating compounds.

Regulatory Pathways of Autophagy Detection

Autophagy dependent on mTOR pathway

PI3K-AKT-mTOR signaling pathway AMPK-TSC 1/2-mTOR signaling pathway

Other signal pathways

3-Methyladenine (3-MA) inhibits autophagy by inhibiting the activity of Class Ⅲ PI3K. beclin1 and UVRAG act as positive regulators, and the anti-apoptotic factor bcl-2 acts as a negative regulator to form a Class III PI3 complex to regulate autophagy. GTP-bound G protein subunit Gαi3 inhibits autophagy; GDP-bound Gαi3 protein activates autophagy. Death-associated protein kinase (DAPK) and DAPK-related protein kinase-1 (DRP-1) induce autophagy.

Project Process

Creative Bioarray has rich research experience in autophagy research. Relying on a professional equipment platform and an experienced scientific research team, we can guarantee the high-quality completion of your project.

Why Choose Us

Professional Platform and Excellent Team

We have a professional technical platform, equipped with advanced instruments and talented and well-trained experts.

Committed to Quality

We put quality first. We provide high-quality products, services and solutions to support customers worldwide.

World-class Service Capabilities

We provide one-stop service, from order to final report, to provide the best solution for your research. We hope to help you complete your research more easily and efficiently.

Customer-centric

We adhere to a mutually beneficial and win-win cooperation model, pay attention to customer needs and goals, and create the greatest value for customers.

If you are interested in our services, please contact us for more detailed information.