Introduction

Another name for apoptosis is "programmed cell death." and it is a multi-step process to eliminate the body's own cells. Unlike cell death caused by necrosis, apoptosis is a physiological process that is affected by external factors or intracellular factors. Apoptotic cells are morphologically manifested as cell shrinkage. They have obvious characteristics of caspase activation and DNA fragmentation to a specific length, and the cell membrane remains intact during these processes. The cell membrane bulges and vesicles are formed, leading to the production of "apoptotic bodies" engulfed by macrophages or neighboring cells. Unlike necrosis, apoptosis does not trigger an inflammatory response, and neighboring cells remain intact. And change all other "microscopy" to microscopy. Microscopy is a method, microscopy is an instrument. Creative Bioarray provides a variety of detection programs to help you systematically analyze the process of apoptosis.

Solutions For you

Creative Bioarray has a professional apoptosis research platform. Our apoptosis detection methods include but are not limited to:

Basic Testing Solutions

Morphology

  • Microscopy
  • Fluorescence Microscopy observation
  • Transmission electron Microscopy observation

Phosphatidylserine Valgus Analysis (AnnexinV Method)

  • Real-time live cell analysis and detection
  • Flow cytometry
  • Fluorescence microscopy

DNA Fragment Detection

  • TUNEL method
  • Gel electrophoresis
  • DNA Ladder determination
  • Flow cytometry analysis of DNA content

Caspase Activity Detection

  • Caspase-3/7
  • Caspase-8
  • Caspase-9
  • Detection of other apoptosis-related proteins such as PARP protein, etc.

Double Detection (Cell Viability + Apoptosis)

We can detect cytotoxicity marked by cell viability and the apoptosis marked by caspase activation to determine cell death mechanism.

Principle:

  • Cell viability: Use cell-permeable substrates to detect live cell proteases in cells and detect fluorescent signals (400Ex/505Em).
  • Caspase-3/7 cleaves the substrate and releases amino luciferin, which emits light under the catalysis of luciferase.

Triple Detection (Cell Viability + Cytotoxicity + Apoptosis)

Creative Bioarray can simultaneously evaluate cell viability, toxicity and apoptosis events.

Detection of Cell Viability and Cytotoxicity
  • Add reagents containing two peptide substrates during the experiment.
  • The fluorescent peptide substrate (GF-AFC) (which can penetrate the cell membrane) enters the living cells and is sheared to generate a fluorescent signal. The signal intensity is proportional to the number of living cells.
  • The second fluorescent peptide base (bisAAF-R110) (cannot penetrate cell membrane) can detect the activity of dead cell protease.
Apoptosis Detection
A reagent containing a luminescent DEVD peptide substrate for caspase-3/7 and a recombinant thermostable luciferase is added to detect apoptosis.

Project Process

Creative Bioarray has rich research experience in the research of apoptosis. Relying on a professional equipment platform and an experienced scientific research team, we can guarantee the high-quality completion of your project.

Project Process

Why Choose Us

Professional Platform and Excellent Team

We have a professional technical platform, equipped with advanced instruments and talented and well-trained experts.

Committed to Quality

We put quality first. We provide high-quality products, services and solutions to support customers worldwide.

World-class Service Capabilities

We provide one-stop service, from order to final report, to provide the best solution for your research. We hope to help you complete your research more easily and efficiently.

Customer-centric

We adhere to a mutually beneficial and win-win cooperation model, pay attention to customer needs and goals, and create the greatest value for customers.

If you are interested in our services, please contact us for more detailed information.

* For scientific research only

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