Product Name
Total Cholesterol and Cholesteryl Ester Assay Kit (Fluorometric)
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Serum, plasma, animal tissue, cells
Applications
For lipid metabolism research.
More Details
Most of the cholesterol in the blood is in the form of cholesterol esters. In the cholesterol assay protocol, these are hydrolyzed to cholesterol by cholesteryl esterase. Cholesterol is then oxidized by cholesterol oxidase to produce H 2 O 2 , which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
