Product Name
Malate Dehydrogenase Assay
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media).
Applications
For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.
Key Features
Fast and sensitive. Linear detection range (20 uL sample): 0.5 to 65 U/L for 20 min reaction at 37°C.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method of Detection
OD565nm
More Details
Malate dehydrogenase (MDH) is an enzyme that reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are two isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is present in the cytoplasm and plays a key role in the malate-aspartate shuttle that transports malate to the mitochondria. MDH2 is a mitochondrial enzyme involved in the TCA cycle that reversibly converts L-malate to oxaloacetate. Higher MDH activity has been found in some neurodegenerative diseases such as Alzheimer's disease.
