Product Name
Isocitrate Assay Kit
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Applications
For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Key Features
Fast and sensitive. Linear detection range (20 sample): 20 to 5000 for 10 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method of Detection
OD565nm
More Details
Isocitrate is an intermediate product of the TCA cycle and is formed from citrate via aconitase activity. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate and produces NAD(P)H. In plants and lower organisms, isocitrate is the branching point of the glyoxalate shunt. Isocitrate is present in fairly high concentrations in many fruits and vegetables and in foods produced from these ingredients.
