Caspase-1 Antibody

Product Category:
Cell Ferroptosis Assay Kit

Description

Our GSH/GSSG assay kit is designed to accurately measure total glutathione, reduced glutathione and oxidized glutathione in biological samples using an enzymatic method utilizing Ellman's reagent (DTNB) and glutathione reductase (GR).DTNB reacts with reduced glutathione to produce a yellow product. The rate of change of optical density measured at 412 nm is proportional to the concentration of glutathione in the sample.

Product Information

Product Number
FEK021
Product Name
GSH/GSSG Assay Kit
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Whole blood, plasma, serum, urine, tissue and cell extracts
Applications
For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).
Key Features
Sensitive and accurate. Linear detection range 0.01-3 uM GSH equivalents with a detection limit of 10 nM GSH equivalents.
Product Type
Assay
Method of Detection
OD412nm
Shelf Life
6 months
Protocol length
20 min
Size
100 tests
Species Reactivity
All
More Details
Glutathione, a tripeptide of glycine, glutamate and cysteine, is one of the key antioxidants that protect cells from damage caused by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteine, where it is converted to the oxidized form GSSG.

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