Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric)

Caspase-1 Antibody

Product Category:
Cell Ferroptosis Assay Kit

Description

Our Coenzyme A assay kit method provides a simple two-step, high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes acyl-CoA to produce enoyl-CoA and H 2 O 2. The resulting H 2 O 2 reacts with a specific dye to form a pink product.

Product Information

Product Number
FEK002
Product Name
Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric)
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Biological
Applications
For quantitative determination of coenzyme A and evaluation of drug effects on coenzyme A metabolism.
Key Features
Sensitive. Use 10 samples. Linear detection range: colorimetric assay 5 - 1000 , fluorimetric assay 3 - 100 CoA. Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.
Product Type
Assay
Method of Detection
OD570nm, or FL530/585nm
Shelf Life
6 months
Protocol length
60 min
Size
100 tests
Species Reactivity
All
More Details
Coenzyme A is involved in many important biological activities, including the synthesis and oxidation of fatty acids and the oxidation of pyruvate in the citric acid cycle. One of the most important roles of coenzyme A is the carriage and transfer of acyl groups.

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