Cholesterol Assay Kit (Colorimetric)

Caspase-1 Antibody

Product Category:
Cell Ferroptosis Assay Kit

Description

Our colorimetric cholesterol assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzing the conversion of cholesterol to cholest-4-ene-3-one, where NAD is reduced to NADH. the optical density of the formed NADH at 340 nm is proportional to the concentration of cholesterol in the sample.

Product Information

Product Number
FEK006
Product Name
Cholesterol Assay Kit (Colorimetric)
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Serum, plasma etc
Applications
For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
Key Features
Sensitive and accurate. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Product Type
Assay
Method of Detection
OD340nm
Shelf Life
6 months
Protocol length
40 min
Size
100 tests
Species Reactivity
All
More Details
Cholesterol is a sterol and lipid present in cell membranes and transported in the blood of all animals. It is used in the formation of cell membranes and hormones and plays an important role in cell signaling processes. Elevated levels (hypercholesterolemia) are associated with cardiovascular diseases such as atherosclerosis.

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