Product Name
Beta Hydroxybutyrate Assay Kit
Storage Conditions
Store at -20 °C
USAGE
For Research Use Only! Not For Use in Humans.
Samples
Serum, plasma, urine etc
Applications
For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism.
Key Features
Sensitive and accurate. Uses 10 uL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature.
High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.
Method of Detection
OD340nm
More Details
Ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) are produced in the liver mainly by the oxidation of fatty acids and are usually present in the urine and blood at low concentrations. Increased concentrations of ketones in the blood may lead to metabolic acidosis, which is associated with diabetes mellitus, hypoglycemia in children, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism.
